Summary of achievements

Phenotypic characterization

Sl no

Species (nos)

Breeds

1

Cattle -17

Deoni, Ongole, Gir, Umblachery, Bachaur, Dangi, Amritmahal. Khillar, Gaolao, Tho Tho, Gangatiri , Kosali, Binjharpuri, Purnea, Assamese, Nallamalla Poda and  Kathani Cattle

2

Buffalo -4

Jaffarabadi, Nagpuri, Surti , Dharwadi

3

Sheep -11

Changthangi, Deccani, Mecheri, Mandya, Rampur  Bushair, Banpala, Coimbatore, Chhotanagpuri , Poonchi, Tibetan and Marwari

4

Goat-9

Osmanabadi, Barbari, Attapady, Ganjam, Mehsana , Bhakarwal , Bundelkhandi, Balangir and Sojat Goat

5

Chicken -4

Aseel, Ankleshwar, Hazra, Poonchi Chicken

6

Camel-5

Kutchi Camel, Mewari , Jalori, Marwari and Sindhi Camel

7

Horse-1

Spiti Horse   

8

Mithun-2

Arunachali and Nagaland

9

Donkey-1

Donkey of Rajasthan

10

Pig-1

Assamese

11

Yak-1

Arunachali

12

Duck-2

Assamese and Manipuri Duck

 

Total

58

Genetic characterization

Sl no

Species (nos)

Breeds

1

Cattle- 14

Kangayam, Amritmahal, Umblacherry, Ongole, Hallikar, Krishna Valley, Bargur,  Cattle population of Assam, Ponwar, Kherigarh, Gangatiri, Kenkatha, Siri, Bachaur, Binjharpuri, Purnea cattle,

2

Buffalo -1

Dharwadi buffalo

3

Sheep -12

Patanwadi, Marwari, Dumba, Madras Red, Nilgiri, Mecheri, Coimbatore, Rampur Bushair, Bonpala, Shahabadi, Balangir,  Tibetan

4

Goat-7

Zalawadi, Gohilwadi, Surti, Kanni adu, Sa;lem Black, Goat of Assam, Ganjam goat

5

Chicken -1

Kadaknath, Hazra chicken

7

Horse-1

Kathiwadi

8

Pig-1

Assamese Pig,

9

Camel - 2

Mewari camel, Jalori camel

 

Total

39

 

In situ Conservation

Sl no

Species (nos)

Breeds

1

Cattle – 7

Tharparkar Cattle, Krishna Valley, Ponwar, Kherigarh, Kangayam, Nagori, Rathi, Bargur and Ongole

2

Buffalo -3

Pandharpuri, Jaffarabadi, Toda 

3

Sheep-4

Magra, Nilgiri, Kilakarsal, Poonchi

4

Goat-2

Beetal, Surti

5

Horse-1

Spiti Horse

6

Camel-1

Kharai Camel

7

Chicken -2

Harringhata Black, Ankaleswar chicken

8

Mithun-1

Nagami
 

Total

21

 

Candidate genes:

Various candidate genes for production (DGAT1, GH, GHR, PIT1, Leptin, CAPN1, CAST, Titin, Akirin, etc) reproduction (GDF9, BMP15, BMPR1B, Kiss1, etc.) and endurance (Myostatin, MCT, CD147, IGF, ACTN, ADRB2) were characterized in indigenous breeds. The Indian breed specific SNPs in these genes were identified and associated with traits in buffalo, cattle, goat and horses.  The novel SNPs have been added to the SNP database of various species. Various new loci of PCR-RFLP have been established for genotyping of identified SNPs in large populations.

 

Buffalo Genomics

More than 100 GB DNA sequence data has been generated from one female Murrah buffalo providing nearly 30-fold coverage. The first version of assembly was constructed with Illumina paired end and mate pair short read sequencing using the cattle genome (Btau 4.0 assembly) as a reference. The assembly has read depth of 17-19X. The buffalo assembly represents ~ 91%-95% coverage in comparison to the cattle assembly Btau 4.0. The assembly has 185,150 contigs. The mitochondrial genome is fully covered by a single contig. Whole genome comparison between this assembly and of cattle revealed 52 million mismatches/indels. The present analysis also unveils about 300 structural variants in the buffalo genome.